Well, I'm using it in what I thought was a fairly traditional definition, where "unisex" means "not peculiar to any one sex." I didn't originally use the male/unisex label, though, I picked it up from someone else, and found it useful because we're (VVC) predominantly female, so that is our largest group to accomodate.

In context of my original discussion, and in how I'm using it, "women" would be for those who self-identify as such (regardless of being cisgendered, trans, genderqueer, etc), while men's/unisex would be for anyone, regardless, while making it specifically clear that it was also open to those who male-identified. Any males uncomfortable with that would then have the option of going to another floor.

Basically, anyone can use either, but there would be a visual identifier that the male/unisex would possibly have males present, for anyone that made uncomfortable, and "unisex" for any males that might be made uncomfortable. Given that we only practically have control over two rooms (I wouldn't be comfortable asking the hotel to let us relabel beyond the two), it seemed an option that best covered alternatives, with the least misunderstanding and potential for offense. But as I noted in my first comment about this, it is an area where I'm still feeling my way, and I'm quite open to being corrected.